Effectiveness Curcuma Longa to Prevent Cells Damage in Early Pregnant Mice with Acute Toxoplasmosis

Tigor Peniel Simanjuntak, Mochammad Hatta, Syahrul Rauf, Andi Mardiah Tahir, Irawan Yusuf, Nurpudi Astuti Taslim, Robert Hotman Sirait, Silvia Arin Prabandari

Abstract


Objective: To prove the effectiveness of Curcuma longa to prevent damage  cells  by analyzing  FOXP3 gene expression, TNF-α level, and histopathology of placental tissue.  

Methods: This study was conducted in 25 early pregnant mice, divided in 5 groups (K1-K5). K1-K4 were injected with  tachyzoites. K1 and K2 were intervened with Curcuma longa 125  and 500 mg/kg/day. K3 were intervened with  spiramycin  60 mg/kg/day, K4 were intervened with 0,2 distilled water, and  K5 was not injected and intervened. IgG-IgM levels, FOXP3 mRNA expression, TNF-α level was examined serially, and  placental mice were taken for histopathology examination  7 days after intervention.

Results:  FOXP3 mRNA expression in K1 and K2  increased significantly 7 days after intervention compared to K4 (p < 0.05), however the increased  of  expression in two groups has no significant difference. TNF-α level in K1 and K2 decreased significantly 7 days after intervention compared to K4  (p < 0.05), however the decrease of  level in two groups has no significant difference. Hemorrhagic and necrotic cells were not found in K1 and K2, but  were found in  K3 (75%)  and K4 (100%).

Conclusion: Curcuma longa 125 mg is effective to prevent cells damage in early pregnant mice with acute toxoplasmosis.


Keywords


Curcuma longa, Cells damage, Early pregnancy, FOXP3 gene, TNF-α, Toxoplasmosis

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DOI: http://dx.doi.org/10.24198/obgynia.v2n2.163

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