Perbedaan Morfologi dan Fragmentasi DNA Sperma sebelum dan sesudah Kriopreservasi dengan Metode Slow Cooling di Klinik Aster RSUP Dr. Hasan Sadikin Bandung

Faizal Arif Caropeboka, Tono Djuwantono, Dian Tjahyadi, Jusuf Sulaeman Effendi, Anita Deborah Anwar, Amillia Siddiq

Abstract


Abstrak
Tujuan: Penelitian ini bertujuan untuk mengetahui perbedaan parameter fragmentasi DNA, morfologi sperma pasca proses pembekuan dengan metode slow cooling.
Metode: Penelitian ini merupakan penelitian obervasional analitik dengan pendekatan pre-post design. Subjek penelitian adalah sperma dengan hasil analisis yang normal sesuai dengan standar WHO (n=25). Penelitian dilakukan di Klinik Aster RSUP Dr. Hasan Sadikin Bandung pada bulan Juli hingga Agustus 2017.
Hasil: Setelah proses kriopreservasi, terdapat peningkatan fragmentasi DNA tiga kali lipat (nilai p<0,05) dan terdapat penurunan jumlah morfologi normal sebesar 50% (nilai p<0,05).
Kesimpulan: Terdapat penurunan kualitas sperma pasca proses kriopreservasi dengan metode slow cooling.

Kata kunci: Fragmentasi DNA, morfologi, slow cooling, spermatozoa


Abstract
Objective: Of this study was to compare sperm quality parameters including DNA fragmentation and morphology after cryopreservation with slow-cooling method.
Method: This was an analytical observational study with pre and post design. Subjects were men whose sperm analysis met the WHO criteria of being normal (n=25). The study was conducted at the Aster Clinic of Dr. Hasan Sadikin General Hospital Bandung from July to August 2017.
Results: After cryopreservation, there was a three fold increase of DNA fragmentation (with p value <0.05) and a decrease in morphology 50% (with p value <0.05).
Conclusion: There is a decrease in sperm quality after cryopreservation with slow-cooling method.

Key words: DNA fragmentation, morphology, slow cooling, spermatozoa



Keywords


Fragmentasi DNA, morfologi, slow cooling, spermatozoa

Full Text:

PDF

References


Mazur P. Principles of cryobiology: life in the frozen state.Edisi.: CRC Press; 2004.

Lestari S, Sari T. Fragmentasi DNA Spermatozoa: Penyebab, Deteksi, dan Implikasinya pada Infertilitas Laki-Laki. eJournal Kedokteran Indonesia. 2015.

Storey K, Storey J. Frozen and alive. Scientific American. 1990;263(6):9-27.

Rocha V, Cornish J, Sievers E, Filipovich A, Locatelli F, C Peters ea. Comparison of outcomes of unrelated bone marrow and umbilical cord blood transplants in children with acute leukemia. Blood. 2001;97(10):2962-71.

Santo MD, Tarozzi N, Nadalini M, Borini A. Human sperm cryopreservation: update on techniques, effect on DNA integrity, and implications for ART. Adv Urol. 2012.

Ozkavukcu S, Erdemli E, Isik A, Oztuna D, Karahuseyinoglu S. Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa. J Assist Reprod Genet. 2008;25(8):403-11.

Rahiminia T, Hosseini A, Anvari M, Ghasemi-Esmailabad S, Talebi A. Modern human sperm freezing: Effect on DNA, chromatin and acrosome integrity. Taiwan J Obstet Gynecol. 2017;56(4):472-6.

Mohamed M. Slow cryopreservation is not superior to vitrification in human spermatozoa; an experimental controlled study. Iran J Reprod Med. 2015;13(10):633.

Boitrelle F, Albert M, Theillac C, Ferfouri F, Bergere M, F Vialard ea. Cryopreservation of human spermatozoa decreases the number of motile normal spermatozoa, induces nuclear vacuolization and chromatin decondensation. Journal Androl. 2012;33(6):1371-8.

Tongdee P, Sukprasert M, Satirapod C, Wongkularb A, Choktanasiri W. Comparison of Cryopreserved Human Sperm between Ultra Rapid Freezing and Slow Programmable Freezing: Effect on Motility, Morphology and DNA Integrity. J Med Assoc Thai. 2015;98:S33-42.

Virro M, Larson-Cook K, Evenson D. Sperm chromatin structure assay (SCSA®) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles. Fertil Steril. 2004;81(5):1289-95.

Lemma A. Effect of cryopreservation on sperm quality and fertility. Artificial insemination in farm animals. InTech. 2011.

Agarwal A, Tvrda E. Chapter 5 Slow Freezing of Human Sperm. Cryopreservation of Mammalian Gametes and Embryos. Methods and Protocols. 2017:67-78.

Orief Y, Schultze-Mosgau A, Dafopoulos K, 2005;10(3):171. SA-H. Vitrification: will it replace the conventional gamete cryopreservation techniques. Middle East Fertil Soc J. 2005;10(3):171.

Thomson L, Fleming S, Barone K, Zieschang J-A, Clark A. The effect of repeated freezing and thawing on human sperm DNA fragmentation. Fertil Steril. 2010;93(4):1147-56.

Paula Td, Bertolla R, Spaine D, Cunha M, Schor N, Cedenho A. Effect of cryopreservation on sperm apoptotic deoxyribonucleic acid fragmentation in patients with oligozoospermia. Fertil Steril. 2006;66(3):597-600.

Zribi N, Chakroun N, Euch HE, Gargouri J, Bahloul A, Keskes L. Effects of cryopreservation on human sperm deoxyribonucleic acid integrity. Fertil Steril. 2010;93(1):159-66.

Gavriliouk D, Aitken R. Damage to sperm DNA mediated by reactive oxygen species: its impact on human reproduction and the health trajectory of offspring.Edisi.: Springer; 2015.

Simonenko E, Garmaeva S, Yakovenko S, Grigorieva A, Tverdislov V, A Mironova ea. The influence of the storage temperature and cryopreservation conditions on the extent of human sperm DNA fragmentation. Biophysics. 2016;61(2):267-70.

Vutyavanich T, Lattiwongsakorn W, Piromlertamorn W, Samchimchom S. Repeated vitrification/warming of human sperm gives better results than repeated slow programmable freezing. Asian J Androl. 2012;14(6):850.

Vutyavanich T, Piromlertamorn W, Nunta S. Rapid freezing versus slow programmable freezing of human spermatozoa. Fertil Steril. 2010;93(6): 1921-8.

Moskovtsev S, Lulat A-M, Librach C. Cryopreservation of human spermatozoa by vitrification vs. slow freezing: Canadian experience. Current Frontiers in Cryobiology. 2012.




DOI: http://dx.doi.org/10.24198/obgynia.v1n2.75

Refbacks

  • There are currently no refbacks.


Alamat Redaksi

 

KSM/Dep Obstetri & Ginekologi Fakultas Kedokteran Universitas Padjadjaran RSUP Dr. Hasan Sadikin

Jl. Pasteur No 38 Bandung 40161 

Telp: 0813-2012-9954

Email: obgyniajurnal@gmail.com

 

 

 




Indexed By
         



Web Analytics
View My Stats

Indonesian Journal of Obstetrics & Gynecology Science @2017