Ekspresi Enzim 1 Alfa-Hidroksilase Plasenta yang Rendah sebagai Faktor Risiko Terjadinya Preeklamsia Berat

Florencia Desiree, I Wayan Artana Putra, I Wayan Megadhana, Anak Agung Ngurah Anantasika, Made Darmayasa, I Gde Sastra Winata


Abstract


Abstrak
Tujuan: Untuk mengetahui ekspresi enzim 1-alfa-hidroksilase (CYP27B1) pada plasenta yang rendah sebagai faktor risiko terjadinya preeklamsia (PE) berat.
Metode: Penelitian ini merupakan studi kasus-kontrol yang tak berpasangan, dengan total sampel 44 kasus dan kontrol. Sampel berupa plasenta yang diproses secara imunohistokimia, untuk melihat ekspresi enzim berdasarkan histoscore kumulatif (H-score) sebagai ekspresi rendah (H-score <200) atau ekspresi tinggi (H-score >200). Regresi logistik ganda digunakan untuk memperkirakan rasio odds yang disesuaikan (OR) dengan interval kepercayaan 95% (95% CI).
Hasil: Ekspresi enzim 1alfa-hidroksilase plasenta yang rendah merupakan faktor risiko terjadinya PE berat sebesar sembilan kali lebih tinggi dibandingkan dengan ekspresi enzim 1-alfa-hidroksilase yang tinggi pada plasenta (OR 9,148; IK05% 2,072-40,386, p=0,002).
Kesimpulan: Ekspresi rendah 1alfa-hidroksilase plasenta meningkatkan risiko terjadinya PE berat.

Low Expression of 1 Alpha-Hydroxylase Enzyme in The Placenta as Arisk Factor for Preeclampsia with Severe Features

Abstract
Objective: This study aims to determine the low expression of the 1-alpha-hydroxylase (CYP27B1) enzyme in the placenta as a risk factor for severe preeclampsia (PE).
Methods: This study is an unpaired case-control study, with a total sample of 44 cases and controls. Samples were placentas that were immunohistochemically processed, to see enzyme expression based on the cumulative histoscore (H-score) as low expression (H-score <200) or high expression (H-score >200). Multiple logistic regression was used to estimate the adjusted odds ratio (OR) and 95% confidence interval (95% CI).
Results: Low placental 1-alpha-hydroxylase expression was a risk factor for severe PE which was nine times higher than placental 1-alpha-hydroxylase expression (OR 9,148; 05% CI 2.072-40,386, p=0.002).
Conclusions: Low placental 1alpha-hydroxylase expression increases the risk of severe PE.

Key words: 1aplha-hydroxylase, expression, placenta, risk factor, severe preeclampsia



Keywords


1aplha-hydroxylase, expression, placenta, risk factor, severe preeclampsia

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References


Sari, N., Rahayujati, T. and Hakimi, M. Kasus Hipertensi pada Kehamilan di Indonesia. Berita Kedokteran Masyarakat, 32(9), 295 p; 2018.

Hernawati, I. Analisis Kematian Ibu Di Indonesia Tahun 2010 Berdasarkan Data SDKI, Riskesdas Dan Laporan Rutin KIA. Available at: http://www.kesehatanibu.depkes.go.id/wpcontent/uploads/downloads/2011/08; 2011

Singla, P., Parkash, AA., Lal, H., Nanda, S. Benefits of Vitamin D Supplementation in Pregnancy for Prevention of Preeclampsia. International Journal of Pharmacy and Biological Sciences;2012; 2(3):144-150.

Halhali, A, et al. Effects of IGF-I on 1,25-dihydroxyvitamin D3 synthesis by human placenta in culture. Molecular Human Reproduction, 1999;5(8), 771-776.

Díaz, L., Sánchez, I., Avila, E., Halhali, A., Vilchis, F. and Larrea, F. Identification of a 25-Hydroxyvitamin D31α-Hydroxylase Gene; 2000.

Morikawa M, Yamada T, Yamada T, Sato S, Cho K, Minakami H. Effects of nulliparity, maternal age, and pre-pregnancy body mass index on the development of gestational hypertension and preeclampsia; 2022.

Sheen, J., Huang, Y., Wright, J.D., Goffman, D., Alton, M.E.D., Friedman, A.M. Maternal age and preeclampsia outcomes. Am. J. Obstet. Gynecol. 220, S222–S223; 2000. Avaliable from: https://doi.org/10.1016/j.ajog.2018.11.339

Tyas, B.D., Lestari, P., Ph, M.D.M.P.H.D., Ilham, M., Akbar, A. Maternal Perinatal Outcomes Related to Advanced Maternal Age in Preeclampsia Pregnant Women. J. Fam. Reprod. Heal. 2019;13;191–200.

Ukah, U.V., Payne, B., Hutcheon, J., Magee, L., Dadelszen, P. Von. Association between maternal age and adverse outcomes in pre-eclampsia. Pregnancy Hypertens. An Int. J. Women’s Cardiovasc. Heal. 13, S16-49; 2018. Avaliable from: https://doi.org/10.1016/j.preghy.2018.08.128

Xiao, JP., Lu, JX., Yin, YX., Jiang, L., Li, WS., Tao, T. Lower Maternal and Fetal Vitamin D Status and Higher Placental and Umbilical Vitamin D Receptor Expression in Preeclamptic Pregnancies. Int J Clin Exp Pathol 2017;10(11):10841-10851

Cooke, C.M., Davidge, S.T., 2021. Advanced maternal age and the impact on maternal and offspring cardiovascular health. Avaliable from: https://doi.org/10.1152/ajpheart.00045.2019

Xiao, JP., Lu, JX., Yin, YX., Jiang, L., Li, WS., Tao, T. Lower Maternal and Fetal Vitamin D Status and Higher Placental and Umbilical Vitamin D Receptor Expression in Preeclamptic Pregnancies. Int J Clin Exp Pathol 2017;10(11):10841-10851

Tubbergen, P., Lachmeijer, A.M.A., Althuisius, S.M., Vlak, M.E.J., Geijn, H.P. Van, Dekker, G.A. Change in paternity: a risk factor for preeclampsia in multiparous women? 45, 81–88; 1999.

Hewison, M., Zehnder, D., Bland, R. and Stewart, P. 1alpha-Hydroxylase and the action of vitamin D. Journal of Molecular Endocrinology, 141-148 p. American College of Obstetricians and Gynecologists. Hypertension in pregnancy. ACOG; 2013.

Hyppönen, E., Cavadino, A., Williams, D., Fraser, A., Vereczkey, A., Fraser, WD. Vitamin D and Pre-Eclampsia: Original Data, Systematic Review, and Meta-Analysis. Ann Nutr Metab 2013;63: 331-340

Tamblyn, J., Susarla, R., Jenkinson, C., Jeffery, L., Ohizua, O., Chun, R., Chan, S.Y., Kilby, M., Hewison, M., 2017. Dysregulation of maternal and placental vitamin D metabolism in preeclampsia. Placenta. Avaliable from: https://doi.org/10.1016/j.placenta.2016.12.019.

Barrera, D., Díaz, L., Noyola-martínez, N., Halhali, A., 2015. Vitamin D and Inflammatory Cytokines in Healthy and Preeclamptic Pregnancies. Nutrients 7, 6465–6490. Avaliable from: https://doi.org/10.3390/nu7085293




DOI: http://dx.doi.org/10.24198/obgynia/v5n2.399

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