Hubungan Ekspresi Cyclooxygenase-2 (Cox-2) dengan Invasi Limfovaskuler, Infiltrasi Sel Radang dan Stadium Kanker Serviks

Andi Ahmed Onterio; Syahrul Rauf; Nugraha Utama Pelupessy; Rina Masadah; Isharyah Sunarno; Nasrudin Andi Mappaware; Sharvianty Arifuddin

doi:10.24198/obgynia.v6i2.478

Hubungan Ekspresi Cyclooxygenase-2 (Cox-2) dengan Invasi Limfovaskuler, Infiltrasi Sel Radang dan Stadium Kanker Serviks

Andi Ahmed Onterio, Syahrul Rauf, Nugraha Utama Pelupessy, Rina Masadah, Isharyah Sunarno, Nasrudin Andi Mappaware, Sharvianty Arifuddin

Abstract

Tujuan: Penelitian ini bertujuan untuk mengetahui hubungan Ekspresi COX-2 dengan Lymphovascular Space Invasion (LVSI), Infiltrasi sel radang dan stadium kanker serviks.
Metode: Penelitian ini menggunakan desain cross sectional di Rumah Sakit Pendidikan Tinggi Negeri Universitas Hasanuddin. Pengambilan sampel menggunakan teknik total sampling dengan data pemeriksaan kadar COX-2 pada 50 hasil biopsi dan hasil operasi pada kasus kasus kanker serviks melalui pemeriksaan immunohistokimia. Data dianalisis dengan uji chi-square dengan tingkat kepercayaan 95%.
Hasil: Hasil penelitian diperoleh ada hubungan signifikan antara eskpresi COX-2 dan stadium kanker serviks dengan nilai p<0,05. Ekspresi COX-2 tidak berhubungan signifikan dengan Lymphovascular Space Invasion dan infiltrasi sel radang dengan nilai p>0,05. Ekspresi COX-2 juga tidak berhubungan signifikan dengan paritas, pendidikan, pekerjaan, penggunaan kontrasepsi oral kombinasi ≥5 tahun, mitra seksual, dan riwayat merokok dengan nilai p>0,05.
Kesimpulan: Berdasarkan hasil penelitian dapat disimpulkan bahwa semakin tinggi stadium kanker serviks maka semakin tinggi ekspresi COX-2, sehingga pemeriksaan ekspresi COX-2 dapat dijadikan sebagai pertanda keganasan pada serviks, selain itu ditemukan LVSI dan infiltrasi sel radang tidak memiliki pengaruh terhadap ekspresi COX-2.

The Correlation Cyclooxygenase-2 (Cox-2) Expression and Lymphovascular Invasion, Inflammatory Cell Infiltration and Cervical Cancer Stage

Abstract
Objective: This study is objected to find out the correlation between COX-2 expression and Lymphovascular Space Invasion, inflammatory cell inflation and cervical cancer stage.
Method: This study uses cross sectional design at educational hospital of Hasanuddin University. COX-2 level examination was performed on 50 biopsy and surgery result on cervical cancer cases through immunohistochemistry. Data were analyzed using chi-square test with significant level of 95%.
Results: Study result indicated that significant correlation is found between COX-2 expression and cervical cancer stage with p value < 0.05. No significant correlation is found between COX-2 expression and Lymphovascular Space Invasion and inflammatory cell inflation with p value > 0.05. No correlation also is found between COX-2 expression and parity, education, profession, combination oral contraception uses 5 years, sexual partner and smoking history with p value > 0.05.
Conclusion: Based on our results it can be concluded that the higher the stage of cervical cancer in line with the expression of COX-2 and examination of COX-2 expression can be used as a marker of cervical malignancy, besides that LVSI and inflammatory cell infiltration not affected on COX-2 expression.

Key words: Cyclooxygenase-2, Cervical Cancer, Stage.

Keywords

Cyclooxygenase-2, kanker serviks, stadium

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References

GBD 2019 Cancer Risk Factors Collaborators. The global burden of cancer attributable to risk factors, 2010-19: a systematic analysis for the Global Burden of Disease Study 2019. Lancet 400, 563–591 (2022).

Dinas Kesehatan Provinsi Sulawesi Selatan. Profil Kesehatan. (2019).

Taylor K. Screening for early cervical cancer. Radiology; 192(1):1-10. (1994).

Gisterek I, Frydecka I, Swiatoniowski G, Fidler S, Kornafel J. Tumour-infiltrating CD4 and CD8 T lymphocytes in breast cancer. Rep Pract Oncol Radiother. 13:205-8. (2008).

Ferrandina, G. et al. Expression of cyclooxygenase-2 (COX-2) in tumour and stroma compartments in cervical cancer: clinical implications. Br J Cancer 87, 1145–1152 (2002).

Divvela, A. K. C., Challa, S. R. & Tagaram, I. K. Pathogenic Role of Cyclooxygenase-2 in Cancer. JOURNAL OF HEALTH SCIENCE 56, 502–516 (2010).

Kulkarni, S. et al. Cyclooxygenase-2 is overexpressed in human cervical cancer. Clin Cancer Res 7, 429–434 (2001).

Subandini, S. R. & Gondhowiardjo, S. A. Ekspresi Siklo-oksigenase-2 (COX-2) pada Kanker Serviks: Tinjauan Khusus Hubungannya dengan Terjadinya Kekambuhan Lokal.

Hoellen, F. et al. Expression of cyclooxygenase-2 in cervical cancer is associated with lymphovascular invasion. Oncol Lett 12, 2351–2356 (2016).

Mandic A, Knezevic SU, Kapiel TI, Malenkovic G. Cyclooxygenase-2 Expression in Cervical Cancer. Oncology Institute of Vojvodina. Serbia :Vojnosanit Pregl 997-1005. (2014).

Klimek, M. et al. Role of cyclooxygenase-2 in cervical cancer. Arch Med Sci 5, 303–307 (2009).

Tekalegn, Y. et al. High parity is associated with increased risk of cervical cancer: Systematic review and meta-analysis of case-control studies. Womens Health (Lond) 18, 17455065221075904 (2022).

World Health Organization. Comprehensive cervical cancer control: a guide to essential practice. (World Health Organization, 2014).

Brito-Marcelino, A., Duarte-Tavares, R. J., Marcelino, K. B. & Silva-Neto, J. A. Cervical cancer related to occupational risk factors: review. Rev Bras Med Trab 18, 103–108 (2020).

Frumovitz. Invasive cervical cancer: Epidemiology, risk factors, clinical manifestations, and diagnosis. (2020).

Finn, O. J. & Edwards, R. P. Human Papillomavirus Vaccine for Cancer Prevention. (2009) doi:10.1056/NEJMe0907480.

Su, B. et al. The relation of passive smoking with cervical cancer. Medicine (Baltimore) 97, e13061 (2018).

Listinawati, L., Sulastri, H., Wresnindyatsih, W. & Azhar, M. B. Hubungan antara Ekspresi Cyclooxygenase-2 dengan Derajat Histopatologi dan Invasi Limfovaskular Karsinoma Sel Skuamosa Serviks. Majalah Kedokteran Sriwijaya 46, 211–215 (2014).

Du, W. et al. Preoperative Prediction of Lymphovascular Space Invasion in Cervical Cancer With Radiomics –Based Nomogram. Frontiers in Oncology 11, (2021).

Naghshvar, F., Torabizadeh, Z., Emadian, O., Enami, K. & Ghahremani, M. Correlation of cyclooxygenase 2 expression and inflammatory cells infiltration in colorectal cancer. Pak J Biol Sci 12, 98–100 (2009).

Huang, M., Chen, Q., Xiao, J., Liu, C. & Zhao, X. Prognostic significance of cyclooxygenase-2 in cervical cancer: a meta-analysis. Int J Cancer 132, 363–373 (2013).

Saldivar, J. S., Lopez, D., Feldman, R. A., Tharappel-Jacob, R., de la Rosa, A., Terreros, D., & Baldwin, W. S. COX-2 overexpression as a biomarker of early cervical carcinogenesis: A pilot study. Gynecologic Oncology, 107(1 SUPPL.). (2007).

Martey, C. A. et al. Cigarette smoke induces cyclooxygenase-2 and microsomal prostaglandin E2 synthase in human lung fibroblasts: implications for lung inflammation and cancer. Am J Physiol Lung Cell Mol Physiol 287, L981-991 (2004).

García-Quiroz, J., Vázquez-Almazán, B., García-Becerra, R., Díaz, L. & Avila, E. The Interaction of Human Papillomavirus Infection and Prostaglandin E2 Signaling in Carcinogenesis: A Focus on Cervical Cancer Therapeutics. Cells 11, 2528 (2022).

Ebrahim, A. H. et al. Galectins in cancer: carcinogenesis, diagnosis and therapy. Ann Transl Med 2, 88 (2014).

DOI: http://dx.doi.org/10.24198/obgynia.v6i2.478